Raman Spectroscopy: A Promising Technique for Analyzing Nucleic Acid Refractory to PCR Amplification
نویسندگان
چکیده
منابع مشابه
Detection of DNA Sequences Refractory to PCR Amplification Using a Biophysical SERRS Assay (Surface Enhanced Resonant Raman Spectroscopy)
The analysis of ancient or processed DNA samples is often a great challenge, because traditional Polymerase Chain Reaction - based amplification is impeded by DNA damage. Blocking lesions such as abasic sites are known to block the bypass of DNA polymerases, thus stopping primer elongation. In the present work, we applied the SERRS-hybridization assay, a fully non-enzymatic method, to the detec...
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ژورنال
عنوان ژورنال: The Open Spectroscopy Journal
سال: 2014
ISSN: 1874-3838
DOI: 10.2174/1874383801408010009